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    Gold dots (50nm)  Gold cylinders  Microcontact Printing  Surface patterns of DsRed-FT by µCP    
 
   
   
     
 

Surface patterns of DsRed-FT by µCP

 
Collaboration: Maryana Escalante, Cees Otto, Jurriaan Huskens, Vinod Subramaniam
(MESA+ Institute for Nanotechnology - University of Twente, Enschede, The Netherlands)
 
   
 
 
 Observation of pattern morphology
 
 Determination of their shape, homogeneity, roughness...
 
 Large field of view
   
 
 
altimage
 
 
       
 
 
 Standard surf SiO2
 
 Optical Microscope with DIC mode
   
   
     
 
As with all the visible fluorescent proteins (VFPs), DsRed and its variants are a popular protein label and marker of gene expression. DsRed is a tetrameric protein composed of monomers of  ~25 kDa mass. µCP was used to immobilize DsRed onto a surface-treated surf.
(Scale bar: 20µm)
 
     
       
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