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Gold dots (50nm) |
| Gold cylinders |
| Microcontact Printing |
| Surface patterns of DsRed-FT by microCP |
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Surface patterns of DsRed-FT by microCP |
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Collaboration: Maryana Escalante, Cees Otto, Jurriaan Huskens, Vinod Subramaniam
(MESA+ Institute for Nanotechnology - University of Twente, Enschede, The Netherlands) |
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| |  | | Observation of pattern morphology | | | | | Determination of their shape, homogeneity, roughness... | | | | | Large field of view | | | | |
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| |  | | SiO2 Surf
| | |  | | Your Optical Microscope : upright, reflected light (BF challenging, DIC mode for guaranteed success) | | | | |
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As with all the visible fluorescent proteins (VFPs), DsRed and its variants are a popular protein label and marker of gene expression. DsRed is a tetrameric protein composed of monomers of ~25 kDa mass. µCP was used to immobilize DsRed onto a surface-treated surf.
(Scale bar: 20µm) |
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