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Spot of a DNA Biochip |
| SiO2 surface structured by optical lithography and coated with epoxy silane and DNA |
| SiO2 surface structured by optical lithography and coated with epoxy silane and DNA |
| SiO2 surface structured by optical lithography and coated with epoxy silane and DNA |
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Specific antibody recognition |
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| Collaboration: V.Souplet, R.Desmet, O.Melnyk (IBL, Lille, France) |
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| |  | | High sensibility | | | | | No labelling | | | | | Non-contact / non-invasive technique | | | | |
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| |  | | SiO2 Surf
| | |  | | Your Optical Microscope : upright, reflected light (DIC mode) | | | | |
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Tag peptides derived from influenza hemagglutinin (HA) and myc protein (myc) were printed on 2 nm-amine-modified standard Surf. Anti-HA antibody on peptide HA (figure A) and anti-myc antibody on peptide myc (figure D) clearly display a spot (about 2nm) whereas no significant change could be observed for the HA microspot in the presence of anti-myc antibody (figure B) and for the myc microspots in the presence of the anti-HA antibody (figure C).
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