Bandeau Technology

Benefits

Among the characterization techniques, the SEEC Microscopy is the only one to provide the visualization and the mapping of nanometric samples in air and in water:

  •  in real-time
  •  without any labeling
  •  with a sub-micrometric lateral resolution (<300nm)

These main features make the SEEC Microscopy a powerful and perfectly adapted technique for the live characterization of any organic and biological samples.

 

Imaging conditions and operation

  • Real-time image
  • Requires NO scanning be it along the x, y or z axis
  • Samples can be observed in air or in aqueous solution
  • Real-time image
  • Straightforward (simply focus and image)
  • True non-contact, non-invasive, non-destructive method
  • Label-free technique. No risk of sample perturbation.
Practical advantages
  • Quickly take snapshots of your samples and post-process your 2D images to get qualitative and some quantitative information
  • Record videos to follow dynamic events and measure the kinetics of a variety of phenomena (wetting, dewetting, evaporation, crystallisation, brownian motion, etc.)
  • No need for advanced training, start getting the most of your SEEC slides right away
  • Pick the aqueous medium that suits best your sample or the phenomenon you are interested in
  • No damage or perturbation to your samples. Observe them as they are, observe the way they behave without interfering

 

 

Extreme sensitivity

  • 1D nano-objects (films) down to 0.1 nm
  • 2D nano-objects (tubes/wires) down to 2 nm across
  • 3D nano-objects (particles) down to 10 nm across
  • Sub-micrometric lateral resolution (<300 nm)
  • Transparent materials differing in refractive index
Practical advantages
  • Use a simple optical systems (ie.light microscope or digital camera) to get high-contrast image of your sample with nanometric details
  • Tell apart tiny transparent objects/regions (within cells, for instance) characterised by a slightly different refractive index or optical thickness

 

 

Compatibility

  • Any optical configuration (DIC, Crossed-polarised, Bright Field...)
  • Labelling techniques (fluorescence, TIRF, ...)
  • Analytical techniques (Raman, FTIR-ATR, ...)
  • Scanning Probe Microscopy (AFM...)
  • Other optical techniques (Confocale...)
Practical advantages
  • Get richer, more thorough information about your sample on a unique platform when possible without extending the duration of your experimental session
  • Biologists fond of fluorescence will be able to use their favourite technique with extra sensitive microscopy or compare their fluorescence images with the super-detailed ones obtained on label-free, samples-on-SEEC slides
  • Chemical analysts such as Raman users will find it particularly handy to be able to shoot their laser beam exactly where the target is rather than be reduced to dealing with a spectrum which mostly if not solely reflects the presence of the substrate's material
  • SPMists will be given the chance to check their samples' uniformity, pick regions of interest, defects or singularities to be mapped in greater lateral details or to be analysed for elasticity, adhesion, friction or any other physical property an SPM (AFM) can probe.

 

 

SEEC Measurement

  • Sample thickness measurement down to 0.3 nm
  • Repetability: better than 0.2 nm (based on 30 measurements, ISO standard 17025)
  • Calibration standard certified by German Metrology Institute (PTB)
  • Fast and easy access to sample thickness measurement (Calibration procedure less than 1 min. then real-time acquisition)
  • Perform thickness measurement in aqueous solution...
  • No risk of damage your samples
  • Ability to get 4D video (3D view as a function of time)
Practical advantages
  • Get easy and fast thickness measurements of your samples
  • Follow in real-time the thickness evolution of your samples
  • Perform precise measurement of soft material such as biological or organic samples
  • Perform kinetic, adsorption/desorption analyses in real-time
  • Compared sample thickness in air and in water
  • Combine analyses with SPM measurements to get information on optical index, density of your samples

 

 

Large and selectable field of view

  • As an example, 2.5x objective: roundabout 4 mm x 3 mm field of view
  • 100x objective: roundabout 100 µm x 75 µm field of view
  • A turret would carry several objectives
Practical advantages
  • Get an overview of your sample at the nanoscale along the z axis and at various lateral scales from the macro- through the meso- to the microscale. Should your optical equipment come with a turret, it is easy to go from one to the next and back
  • Check the sample's uniformity, pinpoint defects, locate isolated micro-targets otherwise tough to find with a near-field imaging technique

 

 

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