PLUG-IN SARFUS 3D

Nanolane develops a software program that transforms an optical microscope into a new nano-tool for ultra-thin films characterization. As fast and simple as your optical microscope. As powerful in z dimension as an AFM .
	Above all, the “Measurement Plug-In” is the only solution which enables you to study dynamic phenomenon in 3D at the nanometer scale, thanks to Sarfus technology. With the “Measurement Plug-In” and its software package, you manage your complete experiment, from the images acquisition to the presentation of your results.

PRODUCT DATA

The Plug-in SARFUS 3D includes :
>>		An acquisition system (digital 3-CCD Camera)
>>		A calibration standard (7steps; 1-50 nm)
>>		A surf support

and SARFUSOFT, the software piece that we develop, that allows :
>>		to control the camera
>>		to acquire optical images (time-lapse function available)
>>		to convert the color image (2D) into metrological image (3D)
>>		to use the Mountains© external software in order to analyse 3D images

The 3 available versions differ according to the functionalities offered by Mountains©
>>		SARFUSOFT 2.0 ST Standrad version: 3D representation, profile thickness, many filters, thresholding...
>>		SARFUSOFT 2.0 XT ST version + management of non-measured points on 3D, series of profiles, fractal analysis, spectral analysis, surface subtraction.
>>		SARFUSOFT 2.0 PR XT version + series of surfaces, (B7/SKIN) vectorisation of the microvalleys network, binary masking, contour analysis, morphological filtering…

4 STEPS ONLY TO GET 3D IMAGES

Step 1: Images acquisition
Step 2: Images correction
Step 3: Images calibration
Step 4: 3D images exploitation


		Your sample was deposited on a Surf and you have adjusted your microscope for a Sarfus observation. The software displays the real-time continuous image coming from the camera. A control panel allows you to manage all the camera parameters. 2 acquisition modes are available: Manual acquisition, to capture individual images Sequential acquisition (Time Laps), which allows capturing slow or fast kinetics. In addition to your sample images, you have to acquire an image of a calibration standard (see calibration standard in product) and another one of a surf background, in the exact same conditions (i.e. light intensity, field or opening diaphragms, acquisition time,…).

Any variation of lighting implies a variation of the colours detected by the camera. Because it is impossible to get a perfectly homogenous lighting on an image taken with a conventional optical microscope, the sample and the calibration standard images must be corrected. Sarfusoft proceeds to this operation by using the image of the surf background.


		The Images calibration is the main Sarfusoft process. It allows the user to perform a construction of the altitude calibration curve which leads to the conversion of a two-dimensional image into a three-dimensional image. In order to do this, we just need to define the thicknesses corresponding to each colour area of the calibration standard.

		The Z-scale construction principle consists in interpolating the discrete values obtained with the image of the calibration standard (7 steps in the calibration standard = 7 altitudes and 7 colours) to be able to allot an altitude to each sample pixel of the images to convert. Topographic images are generated automatically.


Sarfusoft creates 3D files to be used with the Moutains© Application in order to let you explore your topographic images. The software will meet all your requirements in terms of surface analysis and 3D representation.

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