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Lipid layer spreading |
| Lectin layer in water (12nm) |
| Nanometric structures of lipid vesicles |
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Lectin layer in water (12nm) |
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| Collaboration: ZH.Huang, MP Valignat (INSERM, Université Aix-Marseille, France) |
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| |  | | Work in immersion | | | | | Non-contact / non-invasive technique | | | | | No labelling | | | | |
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| |  | | Immersion surf | | |  | | Optical Microscope with DIC mode | | | | |
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Immersion Surf (top layer: SiO2) has been firstly cleaned with piranha and UV lighting before silanisation (mercapto propyltrimethoxysilane). Then, lectin-PEG molecules have been grafted on the mercapto-silane surface. The thickness of the lectin layer is about 12nm and its density, determined by confocal microscopy, is about 500 lectins/µm². The homogeneity and cleanliness of the layer is characterized.
(scale bar: 30µm) |
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