Bandeau Applications

Life Sciences

Access to other applications in Life Sciences

Biochips

Spot of a DNA Biochip
SiO2 surface structured by optical lithography and coated with epoxy silane and DNA
SiO2 surface structured by optical lithography and coated with epoxy silane and DNA
SiO2 surface structured by optical lithography and coated with epoxy silane and DNA
SiO2 surface structured by optical lithography and coated with epoxy silane and DNA
Specific antibody recognition
Sarfus 3D image of a DNA spot in a biochip
PLL-g-PEG biotin chip
PLL-g-PEG biotin chip (3D)
Protein-protein interaction : PLL-g-PEG biotin and streptavidin

Application Notes

SARFUS : Study of chemical micropatterning on polycarbonate for peptide immobilization

A new way is described to micro pattern polycarbonate surface for biomolecular interaction studies. The Sarfus technique allows characterizing the micropatterns and reveals a doughnut effect which hopefully has a minor effect on the fluorescence image.

SEEC Microscopy : Direct detection of biomolecular interactions

Interaction between antibodies and peptide microspots are studied by SEEC Microscopy. Before incubation, measurement shows that the microspot thickness increases versus the concentration of the printed peptide solution. Incubation with antibodies results

Publications

Real-Time Monitoring of Dextransucrase-Based Enzymatic Reaction in Liquid

AMC.Egea, M.Metivier, P.Croguennoc, M.Remaud-Simeon, C.Vieu, BioNanoSci., March 2014,Vol.4, pp 37-45

"Chemical Micropatterning of Polycarbonate for Site-Specific Peptide Immobilization and Biomolecular Interactions"

Olivier Carion, Vianney Souplet, Christophe Olivier, Céline Maillet, Nicolas Medard, Ouafâa El-Mahdi, Jean-Olivier Durand, and Oleg Melnyk ChemBioChem 2007, 8, 315 - 322

"Imaging of protein layers with an optical microscope for the characterization of peptide microarrays"

Vianney Souplet, Rémi Desmet and Oleg Melnyk J. Pept. Sci., 2007, 13, 451 - 457

"Nouvelles méthodologies pour la préparation et la lecture de biopuces à peptides"

THÈSE de Vianney SOUPLET - Chapitre III - "Détection directe d'interactions biomoléculaires avec la technique Sarfus" (in French only) Université du droit et de la santé de Lille 2, France - 28 Février 2007 "Un des buts de nos recherches est d'obtenir u

"Towards Zirconium Phosphonate-Based Microarrays for Probing DNA-Protein Interactions : Critical Influence of the Location of the Probe Anchoring Groups"

Julien Monot, Marc Petit, Sarah M. Lane, Isabelle Guisle, Jean Léger, Charles Tellier, Daniel R. Talham, and Bruno Bujoli J. Am. Chem. Soc., 130 (19), 6243-6251, 2008. 10.1021/ja711427q

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