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Streptavidin (4nm) |
| Streptavidin (4nm) |
| PDMS lithography (7nm) |
| Polymer brushes (75 nm) |
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Protein patterns analysis |
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| Collaboration: A.Ponche, IS2M, Mulhouse, France |
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|  | | Fast and direct visualization for quality-control and defects detection |
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| Large field of view (up to a few mm²) with nanometric sensitivity (down to 0.3nm) |
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Hexagonal patterns of proteins are obtained by microcontact printing of a solution of ovalbumin using a PDMS stamp with hexagonal features (16 microns in diameter). The amount of protein deposited is a function of the concentration of the solution and the duration of inking on the stamp. Pattern quality is checked out in real-time with low magnification objectives (5x and 20x). After calibration, the variation in pattern thickness along a straight line is measured in order to estimate the heterogeneity of the deposition process. |
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