 |
|
|
|
| |
|
 |
|
 |
|
|
|
|
|
|
|  |
| |
|
Lipid layer spreading |
| Lectin layer in water (12nm) |
| Nanometric structures of lipid vesicles |
| |
|
|
 |
|
|
| |
|
| |
|
| |
|
|
| |
Nanometric structures of lipid vesicles |
| |
| Collaboration: Collaboration: Z.H.Huang, M.P.Valignat, INSERM U600, Marseille (France) |
|
|
| |
 |
|
| |
 |
| |  | | Understanding of cellular adhesion | | | | | Direct observation in liquid media | | | | | No labelling | | | | |
|
 |
|
|
|
|
| |
|
|
|
| |
 |
| |  | | Immersion surf | | |  | | Optical Microscope with DIC mode | | | | |
|
|
|
|
|
| |
|
|
| |
 |
A piranha treatment is performed to silanized the surface of the Immersion Surf. Then, a lectin-PEG chain is grafted on the trimethoxysilane surface. The Sarfus image shows a lipid vesicle adsorbed on this pretreated Immersion Surf. Nanometric structures being responsible of the adhesion of the vesicle on the protein surface are studied.
(scale bar: 20 µm) |
|
|
|
|
| |
|
|
| |
|
|
|
|
|
| |
|
|
|
