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Organic steps (6nm) |
| Organic steps (6nm) in 3D |
| Two-Component Phase Patterns in Model Pulmonary Surfactant Systems |
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Two-Component Phase Patterns in Model Pulmonary Surfactant Systems |
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| Collaboration: H. Nakahara & O. Shibata (Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Japan) |
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|  | | Direct visualisation of LB layers at nanoscale |
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| Optical thickness measurement |
| | | Large field of view | | | | |
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|  | | Standard surf |
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| Sarfus 3D |
|  | | Optical Microscope with DIC mode | | | | |
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Two-component monolayers (XHel 13-5 = 0.1) of dipalmitoylphosphatidylcholine (DPPC) and Hel 13-5 (or a mimicking peptide of SP-B protein in pulmonary surfactants) were transferred onto the Surf by the Langmuir-Blodgett (LB) technique [H. Nakahara et al., Langmuir 22 (2006) 1182-1192]. The LB film preparation was made at 35 mN/m on a 0.02 M Tris buffer and 0.13 M NaCl (pH 7.4) at 298.2 K. Considering the molecular thickness and orientation, the higher particle is made of DPPC and the surrounding lower region consists of Hel 13-5. The determined optical height difference of ~0.7 and ~1.1nm provides a good agreement with the previous data obtained by AFM measurements [H. Nakahara et al., Langmuir 22 (2006) 1182-1192].
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